Anirban Bhunia

Anirban Bhunia
Associate Professor

Research interests:

My lab is engaged in the systematic design of several peptides (which are considered as “nano-indicators”) and determine its three-dimensional structure using NMR spectroscopy in combination with molecular dynamics (MD) simulation to correlate its structure with  respective function such as antimicrobial activity, or antifreeze peptide or inhibitors of fibrillation.

Contact:

Address: Department of Biophysics
Centenary Campus
Bose Institute
P-1/12 C.I.T. Scheme VII-M
Kolkata - 700054, India
E-Mail: bhunia[at]jcbose.ac.in
Phone: +91-33-25693336

Research:

Publications:

2017

   74. Datta A, Jaiswal N, Ilyas H, Debnath S, Biswas K, Kumar D, Bhunia A* (2017) Structural   and Dynamic Insights into a Glycine-Mediated Short Analogue of a Designed Peptide in Lipopolysaccharide Micelles: Correlation Between Compact Structure and Anti-EndotoxinActivity. Biochemistry (Just Accepted).

73. Singh S, Datta A, Schmidtchen A, Bhunia A, Malmsten M (2017) Tryptophan end-tagging for promoted lipopolysaccharide interactions and anti-inflammatory effects. Sci. Rep. (Nature Publishing Group) (Paper in press).

72. Korshavn KJ, Satriano C, Lin Y, Zhang R, Dulchavsky M, Bhunia A, Ivanova MI, Lee Y-H, Rosa CL, Lim MH, Ramamoorthy A* (2017) Reduced Lipid Bilayer Thickness Regulates the Aggregation and Cytotoxicity of Amyloid beta. J. Biol. Chem.  (Paper in press).  

71. Ilyas H, Datta A, Bhunia A* (2017) An Approach Towards Structure Based Antimicrobial Peptide Design For Use In Development of Transgenic Plants: A Strategy For Plant Disease Management. Curr. Med. Chem.  (Just Accepted).   (Invited review article)

70. Ghosh A, Pradhan N, Bera S, Datta A, Krishnamoorthy J, Jana NR, Bhunia A* (2017) Inhibition and Degradation of Amyloid Beta (Aβ40) Fibrillation by Designed Small Peptide: A Combined Spectroscopy, Microscopy and Cell Toxicity Study. ACS Chem Neuroscience  (Just Accepted). 

                     2016

69. Manila RS, Ta A, Sinha R, Theeya N, Ghosh A, Tasneem M, Bhunia A,  Koley H, Das S* (2016) Ribavirin suppresses bacterial virulence by targeting LysR-type transcriptional regulators.  Sci. Rep. (Nature Publishing Group)  6, 39454.

68. Ratha BN, Ghosh A, Brender JR, Gayen N, Ilyas H, Neeraja C, Das KP, Mandal AK, Bhunia A* (2016) Inhibition of Insulin Amyloid Fibrillation by a Novel Amphipathic Heptapeptide: Mechanistic Details Studied by Spectroscopy in Combination with Microscopy.  J. Biol. Chem.  291, 23545-23556.

67. Datta A, Yadav Y, Ghosh A, Choi J, Bhattacharyya D, Kar RK, Ilyas H, Dutta A, An E, Mukhopadhyay J, Lee DK, Sanyal K, Ramamoorthy A, Bhunia A* (2016) Mode of Action of A Designed Antimicrobial Peptide : High Efficiency in Killing the Human Fungal Pathogen Cryptococcus neoformans. Biophysical Journal  111, 1724-1737. This article has been selected as "Featured article" as well as "slider" by the journal !!!!        [LINK] 


The article: Biophysical Characterization of Essential Phosphorylation at the Flexible C-Terminal Region of C-Raf with 14-3-3ζ Protein., Plos One, 2015 (DOI: 10.3410/f.725732146.793523456), has been recommended in F1000Prime as being of special significance in its field by F1000 Faculty Member Vladimir Uversky.


66. Pal I, Brahmhatri VP, Bera S, Bhattacharyya D, Quirishi Y, Bhunia A*, Atreya HS* (2016) Enhanced Stability And Activity of an Antimicrobial Peptide in Conjugation With Silver Nanoparticle. J. Colloid Interface Sci., 483, 385-393.

65. Bera S, Kar RK, Mondal S, Pahan K, Bhunia A* (2016) Structural Elucidation of Cell-Penetrating Penetratin Peptide in Model Membranes at Atomic Level : Probing Hydrophobic Interactions in the Blood-Brain-Barrier. Biochemistry  554982-4996.

64. Roy A, Dutta A, Roy D, Ganguly P, Ghosh R, Kar RK, Bhunia A, Mukhopadhyay J, Chaudhuri S* (2016) Deciphering the role of the AT-rich interaction domain and the HMG-box domain of ARID-HMG proteins of Arabidopsis thaliana. Plant Mol. Biol.  Just accepted.

63. Bera S, Korshavn KJ, Kar RK, Lim MH, Ramamoorthy A, Bhunia A* (2016) Biophysical Insights into the Membrane Interaction of the Core Amyloid Forming Ab40 Fragment K16-K28 and its Role in the Pathogenesis of Alzheimer's Disease. Phys Chem Chem Phys.  18, 16890-901.

62. Mitra P, Pal U, Maiti NC, Ghosh A, Bhunia A, Basu S* (2016) Identification of Modes of Interactions Between 9-Aminoacridine Hydrochloride Hydrate and Serum Proteins by Low and High Resolution Spectroscopy and Molecular Modeling. RSC Adv.  6, 53454 - 68.

61. Bhaumik I, Kar RK, Bhunia A, Misra A* (2016) Expedient Synthesis of Pentasaccharide Repeating Unit of the O-antigen of E.coli 086 and Its Conformational Analysis.  Glycoconj. J.  Just Accepted.

60. Datta A, Bhattacharyya D, Singh S, Ghosh A, Schmidtchen A, Malmsten M*, Bhunia A* (2016) Role of Aromatic Amino Acids in Lipopolysaccharide and Membrane Interactions of Antimicrobial Peptides for Use in Plant Disease Control.  J. Biol. Chem.   291, 13301 - 17.

59. Kar RK, Gazova Z, Bednarikova Z, Mroue KH, Ghosh A, Zhang R, Ulicna K, Siebert HC, Nifantiev NE, Bhunia A* (2016) Evidence for Inhibition of Lysozyme Amyloid Fibrillization by Peptide Fragments from Human Lysozyme: A Combined Spectroscopy, Microscopy and Docking Study.  Biomacromolecules   17, 1998 -2009 .

58. Roy P, Dhara D, Parida PK, Kar RK, Bhunia A, Jana K, Sinha-Babu SP, Misra A* (2016) C-Cinnamoyl glycosides as a new class of anti-filarial agents.  Eur. J. Med. Chem.  114, 308-17.

57. Kar RK, Mroue KH, Kumar D, Tejo BA, Bhunia A* (2016) Structure and Dynamics of Antifreeze Protein - Model Membrane Interactions: A Combined Spectroscopic and Molecular Dynamics Study.  J. Phys. Chem. B  120, 902-914.

56. Ghosh A, Bera S, Shai Y, Mangoni, ML, Bhunia A* (2016) NMR Structure and Binding of Esculentin-1a (1‑21)NH2 and Its Diastereomer To Lipopolysaccharide: Correlation With Biological Functions. BBA - Biomembrane  1858, 800-812. 

55. Korshavn KJ, Bhunia ALim MH, Ramamoorthy A* (2016) Ab40 Adopts a Conserved, Partially Folded Structure upon Binding to Zwitterionic Bilayers Prior to Amyloid Formation. ChemComm. 52, 882-5. Selected for Cover page !!!!

54. Pithadia AS, Bhunia A, Sribalan R, Padroni V, Fierce CA, Ramamoorthy A* (2016) Influence of a Curcumin Derivative on hIAPP aggregation in the Absence and Presence of Lipid Membrane. ChemComm. 52, 942-5. This paper has been recommended by Faculty 1000 Prime !!!!

53. Mangoni ML*,  Bhunia A*, (2016) Editorial: Antimicrobial Peptides in Medicinal Chemistry: Advances and Applications. Curr. Top. Med. Chem. 16, 2-3.

52. Sharma S, Sahoo N, Bhunia A*, (2016) Antimicrobial Peptides and their Pore/Ion Channel Properties in Neutralization of Pathogenic Microbes Curr. Top. Med. Chem.16, 42-53.

51. Datta A, Kundu P, Bhunia A* (2016) Designing potent antimicrobial peptides by disulphide linked dimerization and N-terminal lipidation to increase antimicrobial activity and membrane perturbation: Structural insights into lipopolysaccharide binding. J. Colloid Interface Sci., 461, 335-345.

2015

50. Korshavn K, Jang M, Kwak YJ, Kochi A, Vertuani S, Bhunia A, Manfredini S, Ramamoorthy A*, Lim MH* (2015) Reactivity of Metal-Free and Metal-Associated Amyloid-beta with Glycosylated Polyphenols and Their Esterified Derivatives. Nature Sci. Rep.  5, 17842.

49. Kar RK, Bhunia A* (2015) Biophysical and Biochemical Aspects of Antifreeze Proteins: Using Computational Tools to Extract Atomistic Information. Progress Biophys. Mol. Biol., 119, 194-201.

48. Kar RK, Bhunia A* (2015) Will It Be Beneficial to Simulate The Antifreeze Proteins at Ice Freezing Condition or at Lower Temperature? J. Phys. Chem B119, 11485-95.

47Ghosh A, Ratha BN, Gayen N, Mroue KH, Kar RK, Mandal AK*, Bhunia A* (2015) Biophysical Characterization of Essential Phosphorylation at the Flexible C-terminal Region of C-Raf with 14-3-3ζ Protein. PLOS One, 10 (8), e0135976.    This paper has been recommended by Faculty 1000 Prime !!!!

46.  Datta A, Ghosh A, Airoldi C, Sperandeo P, Mroue KH, Barbero JJ, Kundu P, Ramamoorthy A, Bhunia A* (2015)Antimicrobial peptides: Insight into Membrane Permeabilization, Lipopolysaccharide Fragments and Application in Plant Disease Control. Nature Sci. Rep.5, 11951.

45.  Gupta S, Chit JC-Y, Feng C, Bhunia A, Tan S-M, Bhattacharjya S* (2015) An Alternative phosphorylation switch in Integrin b2 (CD18) tail for DOK1 binding. Nature Sci. Rep.5, 11630. 

44. Bera S, Ghosh A, Sharma S, Debnath T, Giri B, Bhunia A* (2015)  Probing the role of Proline in the Antimicrobial activity and lipopolysaccharide binding of Indolicidin. J Colloid Interface Sci.452, 148-159.

43. Ghosh A, Pithadi A, Bhat J, Bera S, Midya A, Fierke C, Ramamoorthy A,  Bhunia A* (2015)  Self-assembly of a 9-residue amyloid-forming peptide fragment of SARS Corona Virus E-protein: Mechanism of self aggregation and amyloid inhibition of hIAPP. Biochemistry54, 2249 -2261

42. Lee DK, Bhunia A, Kotler SA, Ramamoorthy A* (2015)  Antimicrobial peptides and inhibition by cholesterol: A solid-state Nuclear Magnetic Resonance study. Biochemistry54, 1897-907.

41.  Nandi SK, Chakraborty A, Panda AK, Ray SS, Kar RK, Bhunia A, Biswas A* (2015) Interaction of ATP with a small heat shock protein from Mycobacterium leprae: Effect on its structure and function.  PLOS Negl. Trop. Diseas.9, e0003661.

40. Sudheendra US, Dhople V, Datta A, Kar RK, Shelburne CE, Bhunia A*, Ramamoorthy A*  (2014) Membrane Disruptive Antimicrobial Activities of Human beta defensin-3 Analogs. Eur. J. Med. Chem.91, 91-99.

     2014

39. DiMauro MA, Nandi SK, Raghavan CT, Kar RK, Wang B, Bhunia A, Nagaraj RH*, Biswas A* (2014)  Acetylation of Gly1 and Lys2 promotes aggregation of human gD-crystallin. Biochemistry53(46), 7269-82. 

38. Ghosh T, Kar RK, Bhunia A, Misra A* (2014)  Synthesis of the pentasaccharide repeating unit of the O-antigen of E.coli 0175 using one-pot glycosylation and its conformational analysis. Tetrahedron53(46), 7269-82. 

37. Jana M, Kar RK, Bhunia A, Misra A* (2014) Synthesis of the tetrasaccharide repeating unit of the O-antigen of the Escherichia coli O69 strain and its conformational analysis.. RSC Adv.4, 079-84. 

36. Ghosh A, Kar RK, Krishnamoorthy J, Chatterjee S*, Bhunia A* (2014) Double GC:GC Mismatch in dsDNA Enhances Local Dynamics Retaining the DNA Footprint: A High-Resolution NMR Study. ChemMedChem., 9(9), 2052-58.  (invited paper).

35. Ghosh A, Kar RK, Jana J, Saha A, Jana B, Krishnamoorthy J, Kumar D, Ghosh S*, Chatterjee S*, Bhunia A* (2014) Indolicidin Targets Duplex DNA: Structural and Mechanistic Insights Employing Combination of Spectroscopy and MicroscopyChemMedChem9(9), 2059-64   (invited paper). 

34. Dhara D, Kar RK, Bhunia A*, Misra AK*  (2014) Convergent Synthesis and Conformational Analysis of the Hexasaccharide Repeating Unit of the O-Antigen of Shigella flexneri Serotype 1D. Eur. J. Org. Chem.  42014, 4577-84.,

33. Ravichandran Y, Krishnamoorthy J, Ramamoorthy A*, Bhunia A* (2014) Potent gamma-secretase inhibitors/modulators interacts with Amyloid-beta fibrils but do not inhibit fibrillation: A high resolution NMR study. Biochem. Biophys. Res. Comm.447(4), 590 -5.

32. Ghosh A, Datta A, Jana J, Kar RK, Chatterjee C, Chatterjee S*, Bhunia A* (2014) Sequence context induced antimicrobial activity: Insight to Lipopolysaccharide permeabilization. Mol. Biosyst.10(6), 1596-612.

31. Santra A, Si A, Kar RK, Bhunia A*, Mishra AK*  (2014) Linear synthesis and conformational analysis of the pentasaccharide repeating unit of the cell wall O-antigen of Escherichia coli O13. Carbohydr. Res.391, 9-15.    

   2013

30. Saravanan R, Joshi M, Mohanram H, Bhunia A, Bhattacharjya S (2013) NMR structure of temporin-1 ta in lipopolysaccharide micelles: mechanistic insight into inactivation by outer membrane. PLOS One, 8(9), e72718.

29.  Banejee V, Kar RK, Datta A, Parthasarathi K, Chatterjee S, Das KP*, Bhunia A* (2013) Use of Small Peptide Fragment as an Inhibitor of Insulin Fibrillation Process: A Study by High and Low Resolution SpectroscopyPLOS One, 8(8), e72318.

28.  Jana J, Kar RK, Ghosh A, Biswas A, Ghosh S, Bhunia A*, Chatterjee S* (2013) Human Cathelicidin Peptide LL37 Bind Telomeric G-Quadruplex. Mol. Biosys.  9(7):1833-6.

27.  Kar RK, Suryadevara P, Jana J, Bhunia A*, Chatterjee S* (2013) Novel G-quadruple Stabilizing Agents: In-silico Approach and Dynamics. J. Biomol. Str. Dynam. 31: 1497-518.

    2012

26.  Shah SHH, Kar RK, Asmawi AA, Rahman MBA, Murad AMA, Mahadi NM, Basri M, Rahman RNZAR, Salleh AB, Chatterjee S*, Tejo BA*, Bhunia A* (2012) Solution Structures, Dynamics, and Ice Growth Inhibitory Activityof Peptide Fragments Derived from an Antarctic Yeast Protein. PLOS One, 7(11), e49788.             

25. Mohanram H, Nip A, Domadia PN, Bhunia A, Bhattacharjya S. (2012) NMR structure, localization, and vesicle fusion of chikungunya virus fusion Peptide. Biochemistry51(40), 7863-72.

24. Bhunia A, Mohanram H, and Bhattacharjya S. (2012) Structural Determinants of the Specificity of a Membrane Binding Domain of the Scaffold Protein Ste5 of Budding Yeast: Implications in Signaling by the Scaffold Protein in MAPK Pathway. Biochim. Biophys. Acta, 1818(5), 1250-60.

23. Bhunia A*, Bhattacharjya S, Chatterjee, S. (2012) Applications of Saturation Transfer Difference (STD) NMR in Biological Systems. Drug Discov. Today, 17(9-10), 505-13.

              2011

22. Bhunia A, Saravanan R, Mohanram H, Mangoni ML, Bhattacharjya S. (2011) NMR structures and interactions of temporin-1Tl and temporin-1Tb with lipopolysaccharide Micelles: mechanistic insights into outer membrane permeabilization and synergistic activity. J. Biol Chem., 286(7), 24394-406.

         2010

21. Domadia PN, Bhunia A, Ramamoorthy A, Bhattacharjya S. (2010) Structure, Interactions, and Antibacterial activities of MSI-594 derived mutant peptie MSI-594F5A in Lipolysaccharide Micelles: Role of the Helical Hairpin Conformation in Outer-Membrane Permeabilization. J. Am. Chem. Soc., 132 (51), 18417-28.

20. Bhunia A, Vivekanandan S, Eckert T, Burg-Roderfeld M, Wechselberger R, Romanuka J, Bäle D, Kornilov AV, von der Leith C-W, Barbero JJ, Nifantiev N, Schachner M, Sewald N, Lü T, Gabius, H-J, Siebert H-C. (2010) Why the structurally different cyclic peptides can be glycomemtics of HNK-1? J. Am. Chem. Soc, 132, 96-105.

19. Bhunia A, Bhattacharjya S. (2010) Mapping Residue Specific Contacts of Polymyxin B with Lipopolysaccharide by Saturation Transfer Difference (STD) NMR: Insights into Outer-membrane Disruption and Endotoxin NeutralizationBiopolymers, 96, 273-287.

18. Bhunia A, Domadia PN, Torres J, Ramamoorthy A, Bhattacharjya S. (2010) NMR Structure of Pardaxin, a Pore-Forming Antimicrobial Peptide, in Lipopolysaccharide Micelles: Mechanism of Outer membrane Permeabilization. J. Biol. Chem., 285(6), 3883-95.

17. Domadia PNLi YFBhunia AMohanram HTan SMBhattacharjya S. (2010) Functional and structural characterization of the talin F0F1 domain. Biochem. Biophys. Res. Commun. 391(1), 159-65.

16. Rathi S, Bhunia A, Bhattacharjya S. (2010) Role of the Central Hinge Region in the Structure and Activity of Melittin: Designing Non-toxic Antisepsis/Antimicrobial Agents. Biochim. Biophys. Acta, 1798(2), 128-139. (Invited Paper)

      2009

15. Bhunia A, Mohanram H, Domadia PN, Bhattacharjya S. (2009) Designed View More

Recognition:

    Teaching:

    Biomolecular NMR spectroscopy course for MSc -PhD / PhD students

    Present:

    Image Name Designation Department Campus Contact number Email
    profile image Abdul Mohid JRF Biophysics Centenary 25693336 skabdulmohid1992
    profile image Aritreyee Datta SRF Biophysics Centenary 0 aritreyee85@gmail.com
    profile image Bhisma Narayan Ratha SRF Biophysics Centenary 25693336 bhisma.n.ratha@gmail.com
    profile image Dibakar Sarkar JRF Biophysics Centenary 25693336 dibakar
    profile image Dipita Bhattacharyya JRF Biophysics Centenary 25693336 dipita15791@gmail.com
    profile image Humaira Ilyas SRF(I) Biophysics Centenary 25693336 humaira2204
    profile image Karishma Biswas JRF Biophysics Centenary 25693336 karishmabiswas@mail.jcbose.ac.in
    profile image Monalisa Kundu JRF Biophysics Centenary 25693336 kundumolalisa92@gmail.com
    profile image Ranit Pariary JRF Biophysics Centenary ranitpariary@gmail.com
    profile image Shruti Mukherjee JRF Biophysics Centenary 25693336 mshruti.2017@gmail.com

    Former:

    Post Doc
    Dr. Moupriya Nag
    Ph.D in Biophysics (Saha Institute of Nuclear Physics, Kolkata)
    (2016 - Till Date)
    Research Associate-I
    Research area - Cross amyloid interactions at membrane surfaces
    Contact - moupriyanag@gmail.com

    Current Graduate Students

    Anirban Ghosh
    M.Sc. in Organic Chemistry (Calcutta University)
    (2011 - Till Date)
    CSIR Ad-hoc (Senior Research Fellow)
    Research area - Peptide NMR
    Contact - anirban.bapi@gmail.com

    M.S. (Pharm.) in Pharmaco-informatics (NIPER-Hajipur)
    2012 - Till Date)
    CSIR Ad-hoc (Direct-Senior Research Fellow)
    Research area - Molecular dynamics (MD) simulation of antifreeze/ antimicrobial/amyloidognic peptide
    Contact - rajivkar@jcbose.ac.in

    M.Sc. in Biotechnology (KIIT-Bhubaneswar)
    (2012 - Till Date)
    CSIR Ad-hoc (Senior Research Fellow)
    Research area -  Application of de novo designed AMPs in inhibition of human pathogens and treatment of plant diseases
    Contact - aritreyee85@jcbose.ac.in

    Bhisma N. Ratha
    M.S. (Pharm.) in Biotechnology (NIPER-Hajipur)
    2014 - Till Date)
    UGC Ad-hoc (Senior Research Fellow)
    Research area - Chaperone peptide and Structural insights of Myelin associated glycoprotein and its association with ganglosides
    Contact - bhisma.n.ratha@gmail.com

    Swapna Bera
    M.Sc. in Life Sciences (Biophysical Chemistry) (Bose Institute)
    2014 - Till Date)
    UGC Ad-hoc (Junior Research Fellow)
    Research area - Structural insights of amyloid beta peptides in membrane
    Contact - swapna.bera317@gmail.com
    M.Sc. in Life Sciences (Molecular & Cellular Biology) (Bose Institute)
    (2015 - Till Date)
    Institute Fellow  (Junior Research Fellow)
    Research area - Transgenic plant using de novo designed AMPs  and studying their effect on inflammatory and cancer pathway.
    Contact - humairailyas2204@gmail.com

    Sk. Abdul Mohid
    M.Sc. in Life Sciences (Biophysical Chemistry) (Bose Institute)
    (2016 - Till Date)
    Institute Fellow (Junior Research Fellow)
    Research Area - Biophysical study of Antimicrobial Peptide
    Contact
     - skabdulmohid1992@gmail.com

    Monalisa Kundu
    M.Sc. in Chemistry (Burdwan University)
    (2016 - Till Date)
    UGC Ad-hoc (Junior Research Fellow)
    Research area - Self assembling of small peptides, design of inhibitors for Type-2 diabetes
    Contact - kundumonalisa92@gmail.com

    Dipita Bhattacharyya
    M.Sc. in Zoology (Calcutta University)
    (2015 - Till Date)
    UGC Ad-hoc (Junior Research Fellow)
    Research area - Biophysical studies of membrane - Alpha Synuclein interaction
    Contact - dipita15791@gmail.com

    Shruti Mukherjee
    M.Sc. in Zoology (Calcutta University)
    (2016 - Till Date)
    Visiting student
    Research area - Biophyiscal studies of Antimicrobial peptide

    Current Project Students

    Shruti Mukherjee
    M.Sc. in Zoology (Calcutta University)
    (2016 - Till Date)
    Visiting student
    Research area - Biophyiscal studies of Antimicrobial peptide


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