Kaushik Biswas

Kaushik Biswas
Associate Professor

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Previous appointments:

Post Doctoral Research Fellow at the Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA

Research interests:

Understanding the mechanism of tumorigenesis specifically focussing on the role of glycolipids in tumor growth, progression and metastasis.

Specific Research Focus :        

  • How tumor derived Gangliosides modulate tumor growth, progression and metastasis ?

  • Targeted Genome Editing tools (TALEN and CRISPR) to study mechanisms in carcinogenesis, focusing primary on unknown functions of genes in cancer

  • Epigenetic regulation of ganglioside synthase genes in cancer

  • Screening and identification of compounds from natural sources as potential therapeutics against cancer

  • Mechanism of tumor induced immune suppression


Contact:

Address: Division of Molecular Medicine
Centenary Campus
Bose Institute
P-1/12 C.I.T. Scheme VII-M
Kolkata - 700054, India
E-Mail: kaushik[at]jcbose.ac.in
Phone: +91-33-25693217

Research:


We have taken an all round approach towards understanding the basis of aberrant ganglioside expression in various tumors as  well as studying the consequence of such an abnormal expression in carcinogenesis. Gene silencing as well as molecular cloning and over-expression strategies have been utilized to establish the potential role  of tumor derived glycosphingolipids in migration as well as invasion of tumor cells. Gene expression profiling suggests a large number of differentially regulated gene sets (DRGs) in response to GM2-synthase knockdown and mechanistic studies reveal a critical role of the integrin signaling pathway to be involved in GM2 mediated tumor cell migration. With an aim to translate these in vitro findings in vivo, targeted genome editing technology by TALEN is used to define the functional role of GM2 in tumorigenesis. Studies indicate that GM2 is involved in epithelial-mesenchymal transition (EMT) by promoting anoikis resistance in tumors. Studies are under way to elucidate the mechanism of GM2 mediated EMT. Finally, we have initiated a study to find out the basis of over-expression of several ganglioside synthase genes in cancer, currently focusing on the regulation of GM2-synthase gene. Preliminary data suggests that there may be a plausible epigenetic role in the regulation of GM2-synthase gene in cancer. On a different note, we have very recently undertaken a collaborative study, to screen and identify potential anti-inflammatory compounds from natural sources, those by virtue of being anti-inflammatory may be able to protect against chronic inflammation induced disease pathogenesis, like cancer.


 

Publications:

Kundu, M, Mahata, B, Banerjee, A, Chakrabarty, S, Debnath, S, Sinha Ray, S, Ghosh, Z and Biswas, K. Ganglioside GM2 mediates migration of tumor cells by interacting with integrin  and modulating the downstream signaling pathway. Biochim Biophys Acta – Mol Cell Res., 1863 (7 Pt A), 1472-1489 (2016).

Mahata, B and Biswas, K. Generation of stable knockout mammalian cells by TALEN mediated locus specific gene editing. Methods Mol Biol., 1498, 107-120 (2017).

Mahata, B, Banerjee, A, Kundu, M, Bandyopadhyay, U and Biswas, K. TALEN mediated targeted editing of GM2/GD2-synthase gene modulates anchorage independent growth by reducing anoikis resistance in mouse tumor cells. Scientific Reports, 5 : 9048, 1-12 (2015).

Mahata, B, Biswas, S, Rayman, P, Chahlavi, A, Ko, J, Bhattacharjee, A, Li, YT, Li, Y, Das, T, Sa, G, Raychaudhuri, B, Vogelbaum, MA, Tannenbaum, C, Finke, JH and Biswas, K. GBM Derived Gangliosides Induce T Cell Apoptosis through Activation of the Caspase Cascade Involving Both the Extrinsic and the Intrinsic Pathway. PLoS One, 10, 1-19 (2015). 

Parida, PK, Sau, A, Ghosh, T, Jana, K, Biswas, K, Raha, S and Misra, AK. Synthesis and evaluation of triazole linked glycosylated 18β-glycyrrhetinic acid derivatives as anticancer agents. Bioorganic & Medicinal Chemistry Letters, 24, 3865-3868 (2014). 

Biswas, K, Richmond, A, Rayman, P, Biswas, S, Thornton, M, Sa, G, Das, T, Zhang,  R, Chahlavi, A, Tannenbaum, CS, Novick, A, Bukowski, R. and Finke, JH. GM2 Expression in Renal Cell Carcinoma : Potential Role in Tumor Induced T cell Dysfunction. Cancer Res, 66, 6816-6825 (2006).

Biswas, K, Bandyopadhyay, U, Chattopadhyay, I, Varadaraj,  A, Ali, E. and Banerjee, RK. A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical. J Biol Chem, 278, 10993-11001 (2003).

Bandyopadhyay, U, Biswas, K, Sengupta, A, Moitra, P, Dutta, P, Sarkar, D, Debnath, P, Ganguly, C.K. and Banerjee, RK. Clinical studies on the effect of Neem (Azadirachta indica) bark extract on gastric secretion and gastroduodenal ulcer. Life Sci, 75, 2867-2878 (2004).

Biswas, S, Richmond, A, Biswas, K, Ko, J, Ghosh, S, Simmons, M, Rayman, P, Rini, B, Gill, I, Tannenbaum, C.S. and Finke, JH. Elevated levels of select gangliosides in T cells from Renal Cell Carcinoma patients is associated with T-cell dysfunction. J. Immunol, 183(8), 5050-5058 (2009).

Chattopadhyay, I, Bandyopadhyay, U, Biswas, K, Maity, P and Banerjee, RK. Indomethacin inactivates gastric peroxidase to induce reactive-oxygen mediated gastric mucosal injury and curcumin protects it by preventing peroxidase inactivation and  scavenging reactive oxygen. Free Radical Biology and Medicine, 40, 1397-1408 (2006).

Chahlavi, A, Rayman, P, Richmond, AL, Biswas, K, Zhang, R, Vogelbaum, M, Tannenbaum, C, Barnett, G and Finke, JH. Glioblastomas induce T-lymphocyte death by two distinct pathways involving gangliosides and CD70.  Cancer Res, 65(12), 5428-38 (2005).

Raval, G, Biswas, S, Rayman, P, Biswas, K, Sa, G, Ghosh, S, Thornton, M, Hilston, C, Bukowski, R, Finke, JH and Tannenbaum, CS. “TNF-a Induction of GM2 Expression on Renal Cell Carcinomas Promotes T cell Dysfunction. J.Immunol, 178, 6642-6652 (2007).

Rayman, P, Wesa, AK, Richmond, AL, Das, T, Biswas, K, Raval, G, Storkus, WJ, Tannenbaum, CS, Novick, A, Bukowski, R and Finke, JH. Effect of Renal Cell Carcinomas on the Development of Type1 T-Cell Responses. Clinical Cancer Research, 10 (18Pt2), 6360S-6366S (2004).

Chattopadhyay, I, Nandi, B, Chatterjee, R, Biswas, K, Bandyopadhyay, U and Banerjee, RK. Mechanism of antiulcer effect of Neem (Azadirachta indica) leaf extract : effect on H+-K+-ATPase, oxidative damage and apoptosis. Inflammopharmacology, 12, 153-176 (2004).

Biswas, K, Chatterjee, I, Banerjee, RK and Bandyopadhyay, U. Biological activity and medicinal uses of Neem (Azadirachta indica). Current Sci, 82, 1336-1345 (2002).

Bandyopadhyay, U, Biswas, K,  Chatterjee,R, Bandyopadhyay, D, Chattopadhyay, I, Ganguly, CK, Chakroborty, T, Bhattacharya, K and Banerjee, RK. Gastroprotective effect of Neem (Azadirachta indica) bark extract through inhibition of H+-K+-ATPase and scavenging of hydroxyl radical. Life Sci, 71, 2845-2865 (2002).

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Recognition:

    Teaching:

    For Integrated MSc PhD Course

    Cell Biology,

    Molecular & Cellular Biology (Core)

    Biochemistry (Practical)


    Present:

    Image Name Designation Department Campus Contact number Email
    profile image Avisek Banerjee SRF Division of Molecular Medicine Centenary 25693337 avisek
    profile image Barun Mahata SRF Division of Molecular Medicine Centenary 25693321 bmahata1987@gmail.com
    profile image Manjari Kundu SRF(Extended) Division of Molecular Medicine Centenary 25693337 manjari
    profile image Shibjyoti Debnath SRF Division of Molecular Medicine Centenary 25693337 shibjyoti

    Former:





    Barun Mahata, SRF (CSIR)

    Tarun Mahata, Project Assistant

    Group News:

    • Apr 28, 2017 -   Barun Mahata ( SRF) submitted his PhD thesis titled "Study the role and elucidate the mechanism of tumor derived gangliosides in tumor growth, progression and metastasis in syngenic mouse tumor model"
    • Apr  01, 2017 -  Manjari Kundu (ext SRF) defended her PhD thesis Viva Voce Examination
    • Feb 18, 2017 -  Barun Mahata (SRF) awarded the best poster award (1st Prize) at the 20th ADNAT convention held at KIIT, Bhubaneswar during Feb 16-18, 2017
    • Jan 10, 2017 -   Avisek Banerjee (SRF) awarded best poster award at the 3rd International Conference on PCSMM held at Bose Institute, Kolkata during Jan 8-10, 2017
    • Aug 22, 2016 -  Manjari Kundu (ext SRF) submitted her PhD thesis titled "Deciphering the role and elucidating the mechanism of tumor derived gangliosides in tumor cell growth, migration, invasiveness and metastasis"
    • Nov 30, 2015 -  Barun Mahata (SRF) awarded Professor B.B. Biswas Outstanding Student Award 2015