Jayanta Mukhopadhyay

Assistant Professor,

Department of Chemistry

Ph.D (Biochemistry & Biophysics),
Jadavpur University, 2000



Research interest

Transcription is the first step in gene expression where most regulation occurs. RNAP core enzyme together with sigma factor(s) and/or numerous regulator(s) orchestrates the gene expression in bacteria. Our lab seeks to characterize the interactions among RNAP, sigma factors, and regulators required for various gene expressions in Mycobacterium tuberculosis. The proposed work will use integrated biophysical, biochemical and genetic approaches, along with a recombinant in vitro transcription system to address two specific aims:

1. Determine the role of alternative sigma factors in gene regulation of M. tuberculosis

2. Identify and characterize inhibitors of M. tuberculosis gene expression.

 

Group:

 Interested students may contact for JRF positions

Relevant publications

  1. Mukhopadhyay J, Das K, Ismail S, Koppstein D, Jang M, Sarafianos St, Tuske S, Hudson B, Patel J, Jansen R, Höfle G, Arnold E, and Ebright RH (2008).  The RNA polymerase "switch region" is a target for inhibitors. Cell, 135: 295-305.

  2. Pavlova O, Mukhopadhyay J, Sineva E, Ebright RH, Severinov K. (2008) Structure-activity  analysis of Microcin J25. J Biol Chem., 283: 25589- 95.

  3. Mukhopadhyay J, Sineva E, Knight J, Levy RL, and Ebright RH (2004) Antibacterial peptide microcin J25 (MccJ25) inhibits transcription by binding within, and obstructing, the RNA polymerase secondary channel. Mol Cell, 14, 739-751.

  4. Mukhopadhyay J, Mekler V, Kortkhonjia E, Kapanidis AN, Ebright YW, Ebright RH (2003) Fluorescence resonance energy transfer (FRET) in analysis of transcription-complex structure and function. Methods Enzymol, 371, 144-159.

  5. Mekler V, Kortkhonjia E, Mukhopadhyay J, Knight J, Revyakin A, Kapanidis AN, Niu W, Ebright YW, Levy R, Ebright RH (2002) Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex. Cell, 108, 599-614.

  6. Mukhopadhyay J, Kapanidis AN, Mekler V, Kortkhonjia E, Ebright YW, Ebright RH (2001) Translocation of 70 with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA. Cell, 106, 453-63.


Contact


 

jayanta@bic.boseinst.ernet.in

PH:  91-33-2303-1143

Fax: 91-33-2303-6790