Department of Chemistry
Ph.D (Biochemistry & Biophysics),
is the first step in gene expression where most regulation
occurs. RNAP core enzyme together with sigma factor(s) and/or
numerous regulator(s) orchestrates the gene expression in
bacteria. Our lab seeks to characterize the interactions among
RNAP, sigma factors, and regulators required for various gene
expressions in Mycobacterium tuberculosis. The proposed
work will use integrated biophysical, biochemical and genetic
approaches, along with a recombinant in vitro
transcription system to address two specific aims:
1. Determine the role of alternative sigma factors in gene
regulation of M.
and characterize inhibitors of M. tuberculosis gene
students may contact for JRF positions
Das K, Ismail S, Koppstein D, Jang M,
St, Tuske S, Hudson B, Patel J,
Jansen R, Höfle G, Arnold E, and Ebright RH (2008).
polymerase "switch region" is a target for inhibitors.
Cell, 135: 295-305.
Mukhopadhyay J, Sineva E, Ebright RH, Severinov K. (2008)
Structure-activity analysis of Microcin J25. J Biol Chem.,
283: 25589- 95.
Mukhopadhyay J, Sineva E, Knight J, Levy RL, and Ebright RH (2004)
Antibacterial peptide microcin J25 (MccJ25) inhibits
transcription by binding within, and obstructing, the RNA
polymerase secondary channel. Mol Cell, 14,
Mukhopadhyay J, Mekler V, Kortkhonjia E, Kapanidis AN, Ebright
YW, Ebright RH (2003) Fluorescence resonance energy
transfer (FRET) in analysis of transcription-complex structure
and function. Methods Enzymol, 371, 144-159.
Kortkhonjia E, Mukhopadhyay J, Knight J, Revyakin A, Kapanidis
AN, Niu W, Ebright YW, Levy R, Ebright RH (2002)
Structural organization of bacterial RNA polymerase holoenzyme
and the RNA polymerase-promoter open complex. Cell,
Mukhopadhyay J, Kapanidis AN, Mekler V, Kortkhonjia E, Ebright
YW, Ebright RH (2001) Translocation of 70
with RNA polymerase during transcription: fluorescence resonance
energy transfer assay for movement relative to DNA. Cell,